Coding
Part:BBa_K2944001:Design
Designed by: Davindra Singh Group: iGEM19_Concordia-Montreal (2019-10-16)
Glucose oxidase optimized for Saccharomyces cerevisiae
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1
Illegal BamHI site found at 42
Illegal BamHI site found at 352
Illegal XhoI site found at 1916 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 376
Illegal BsaI.rc site found at 1594
Design Notes
The entire sequence was initially separated in two parts for ordering purposes from Twist Bioscience. They were then ligated back together seamlessly, but retain BsaI restriction sites within the sequence.
Source
Taken from BBa_K2238000 then codon optimized.