Coding

Part:BBa_K2944001:Design

Designed by: Davindra Singh   Group: iGEM19_Concordia-Montreal   (2019-10-16)


Glucose oxidase optimized for Saccharomyces cerevisiae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
    Illegal BamHI site found at 42
    Illegal BamHI site found at 352
    Illegal XhoI site found at 1916
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 376
    Illegal BsaI.rc site found at 1594


Design Notes

The entire sequence was initially separated in two parts for ordering purposes from Twist Bioscience. They were then ligated back together seamlessly, but retain BsaI restriction sites within the sequence.


Source

Taken from BBa_K2238000 then codon optimized.

References